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human gingival fibroblasts hgfs  (ATCC)


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    ATCC human gingival fibroblasts hgfs
    Human Gingival Fibroblasts Hgfs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 539 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+gingival+fibroblasts+hgfs/Primary+Gingival+Fibroblast%3B+Normal%2C+Human%2C+Adult/pmc13690205-68-0-8
    Average 99 stars, based on 539 article reviews
    human gingival fibroblasts hgfs - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Antimicrobial DMAHDM nanoparticle-modified resin for 3D printing: Composition-Property relationships.
    Article Snippet: This study aimed to determine the effects of the proportion of dimethylaminohexadecyl methacrylate (DMAHDM) nanoparticle to urethane acrylate (UA)-based 3D-printing resin on antibacterial and antifungal activities, biocompatibility, degree of conversion (DC), and mechanical properties.. UA-based resin and DMAHDM were synthesized separately and mixed to prepare specimens at DMAHDM proportions of 0–1 wt%.. The WST-8 viability assay was used to assess the antibacterial effects against Streptococcus mutans (S. mutans), and the growth of Candida albicans (C. albicans) was measured by OD600 to evaluate antifungal activity.

    Article Title: Nanotechnology-based electrochemical approach for effective root canal irrigation.
    Article Snippet: Root canal treatment (RCT) is an endodontic procedure to preserve an infected/inflamed tooth and retain its function.. Root canal irrigation is the most crucial step in RCT for cleaning the root canal space.. Achieving total disinfection in root canal space is still a significant challenge in dentistry.

    Modification:

    Article Title: Nanotechnology-based electrochemical approach for effective root canal irrigation.
    Article Snippet: Root canal treatment (RCT) is an endodontic procedure to preserve an infected/inflamed tooth and retain its function.. Root canal irrigation is the most crucial step in RCT for cleaning the root canal space.. Achieving total disinfection in root canal space is still a significant challenge in dentistry.

    Purification:

    Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis
    Article Snippet: .. The Est816 was prepared and purified according to our established protocols, and its molecular mass was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis., , Human gingival fibroblasts (HGFs) (HGF-1, ATCC CRL-2014) were used in the following experiments. ..

    Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis.
    Article Snippet: .. The Est816 was prepared and purified according to our established protocols, and its molecular mass was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.9,15,16 Human gingival fibroblasts (HGFs) (HGF-1, ATCC CRL-2014) were used in the following experiments. ..

    Gel Electrophoresis:

    Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis
    Article Snippet: .. The Est816 was prepared and purified according to our established protocols, and its molecular mass was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis., , Human gingival fibroblasts (HGFs) (HGF-1, ATCC CRL-2014) were used in the following experiments. ..

    Electrophoresis:

    Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis.
    Article Snippet: .. The Est816 was prepared and purified according to our established protocols, and its molecular mass was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.9,15,16 Human gingival fibroblasts (HGFs) (HGF-1, ATCC CRL-2014) were used in the following experiments. ..

    Cytotoxicity Assay:

    Article Title: Novel Resin-Based Antibacterial Root Surface Coating Material to Combat Dental Caries
    Article Snippet: .. After receiving approval from the University of Maryland, a cytotoxicity assay was conducted using human gingival fibroblasts (HGFs) (hGFBs, CRL-4061, ATCC) due to their proximity to restorative materials. ..



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    (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts <t>(HGF-1)</t> labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.
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    human gingival fibroblasts  (ATCC)
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    (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts <t>(HGF-1)</t> labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.
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    Image Search Results


    (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts (HGF-1) labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.

    Journal: RSC Advances

    Article Title: Genipin-crosslinked CeO 2 -incorporating Janus patch for oral ulcer treatment

    doi: 10.1039/d6ra00275g

    Figure Lengend Snippet: (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts (HGF-1) labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.

    Article Snippet: Human gingival fibroblasts (HGF-1) were purchased from ATCC (Manassas, USA).

    Techniques: Comparison, Fluorescence, Labeling, Co-Culture Assay, CCK-8 Assay, Staining, Isolation